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However, not every protein of interest is produced in or secreted by P. pastoris to such high titres.
Accordingly, a broadly applicable companion strategy to genome mining that would allow the determination of whether a natural product of interest is produced at a detectable level would be valuable.
The mRNA coding for a gene of interest is produced synthetically (in vitro translation) and is injected at varying concentrations into zebrafish eggs at the 1-2 cell stage; then the translation of the mRNA produces higher amounts of the encoded protein.
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Heatmap profiles for genes of interest were produced by Excel program.
PCR fragments containing the gene of interest were produced from yeast genomic DNA as template.
Contrast images of interest were produced from these individual analyses and used in the second-level higher analysis.
This protocol also assesses whether in-frame or out-of-frame alleles for the gene of interest are produced.
This common interest was produced by what has come to be known as "the serials crisis," a chronic escalation of institutional subscription fees far in excess of inflation.
High-titre, replication-incompetent retroviral particles encoding the RNA of interest were produced in the Phoenix Eco packaging line (Orbigen, San Diego, CA, USA) for murine melanocytes.
By fitting each subject's data to the GLM, 3D parameter estimate maps for each of our conditions of interest were produced.
DNA probes designed on 3'UTR regions of genes of interest were produced by PCR reaction and labeled with [α-P]-dCTP using Prime-a-Gene® Labelling System (Promega, USA) according to manufacturer's protocol.
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