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Thus, the nonstandard translation system of ciliates requires additional research before any protein of interest is expressed because the expression of ciliate proteins in common expression systems using Escherichia coli or yeast will result in premature polypeptide chain termination.
Noteworthy, despite the T47D Tet-On cell line is designed to be an inducible gene expression system, where the gene of interest is expressed only in the presence of doxycycline, PRL was constitutively produced by the T47D/pTRE-PRL clone Cl.4 and no major induction of PRL expression was observed by addition of doxycycline (data not shown).
Almost invariantly, the gene of interest is expressed in a constitutive fashion, although this may not be strictly necessary for several applications.
To this end, the recombinant part of the protein of interest is expressed as a fusion protein with one split intein fragment.
Here we explore nine different chimeric vaccine designs, in which the antigen of interest is expressed as an in-frame fusion with polypeptides that impact antigen processing or presentation.
Therefore, the problem of interest is expressed as: begin{array}{*{20}l} &min_{{boldsymbol{X}_{k} ; t_{k},; alpha}} textstyle alphasum_{m=1}^{M} P_{m} notag text{s.
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They are based on calculating relative expression values: expression levels of genes of interest are expressed relative to the expression of one or a panel of several suitable reference genes.
The values of differential expression of the genes of interest were expressed as has been described previously [ 23].
This study indicates that some groups of men are interested and concerned about their current and future health, albeit that their interest was expressed through concerns about bio-physical issues.
Every time interest was expressed in Rachele, Ralph began tracking down that college's softball roster.
Once again, the linkages of interest are expressed at the higher length scale as well as the lower length scale.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com