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If an abnormal event of interest is detected during the specified operations, then Eqs.
The region of interest is detected and then the signs of selected transform coefficients are scrambled.
Further reconstruction and post processing can be performed once an abnormality or area of interest is detected in these images.
In the first step, the object of interest is detected by the largest sparse coefficient; in the second step, the detected object is validated with a binary classifier based on sparse representation, which outputs the decision in terms of the distribution of sparse coefficients.
In the current version of the tool, the process consists of simulating a dataset with a number of specific characteristics and seeing in what proportion of the simulations the effect of interest is detected.
To ensure that only a sparse subset of fluorophores resides in the on-state at any time of the experiment and that each fluorophore defining the structure of interest is detected as individual emitter and thus precisely localized, the generation of stable off-states with lifetimes exceeding seconds is of outstanding importance.
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The proteins of interest were detected using the ECL Plus detection system (Amersham, Piscataway, NJ).
The blots were washed again and the proteins of interest were detected using Amersham ECL Prime Western Blotting Detection Reagents (GE Healthcare) according to the manufacturer's instructions, and the chemiluminescence signal was then visualized with X-ray film.
The protein of interest was detected using ECL or ECL plus chemiluminescent Western blotting detection reagent and Hyperfilm ECL (Amersham plc., Little Chalfont, UK).
The RNA was blotted onto a positively charged nylon membrane (Roche) according to standard procedures, and the transcript of interest was detected using DIG-labeled antisense probes (DIG labeling and detection, Roche).
In cases when no objects of interest are detected, again classical average metering is performed [17].
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