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On the basis of guidelines concerning the flanking residues of serines/threonines and cysteines, a peptide of interest is designed with serine/threonine and cysteine at appropriate positions to allow their effective participation in cyclization.
The optimal chart which provides the quickest out-of-control detection speed for a specified shift of interest is designed according to the number of Phase-I samples commonly used in practice.
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The problem of particular interest is designing the secondary channel matrix, when the primary channel matrix is fixed.
Primer pairs for the amplification of each gene region of interest were designed by using AmpliSeq Designer v.1.2.6 software [ 8] (Life Technologies).
Specific primers targeting each unique sequence of interest were designed by hand.
Asialofetuin conjugates with DNA oligonucleotides (AF/DNA) complementary to the PNA of interest were designed as cell-specific delivery vectors.
Both genes of interest were designed with restriction site NdeI at the 5′ end and XbaI at the 3′ end, immediately outside of the coding region to facilitate subsequent cloning.
Primers specific to genes of interest were designed as recommended by PrimerBank (www.primerbank.org).org
Validated primers for each gene of interest were designed for each target mRNA (Table 1B).
Primers corresponding to the 11 genes of interest were designed using Primer Express software (version 2.0, Applied Biosystems).
Forward and reverse primers for each gene of interest were designed from separate exon sequences to eliminate possible artefacts due to potential DNA contamination in RNA preps.
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