Exact(2)
Using our Flip-Flop HSV-BAC system, once a shuttle vector containing a promoter of interest is constructed, nearly pure (>99%) preparations of recombinant vector can be obtained typically within two weeks, which can be easily plaque-purified subsequently.
If, on the other hand, a more complex translational reporter, in which the reporter is fused in-frame to the protein-coding sequence of the gene of interest, is constructed then, in addition to deriving information about promoter activity, potentially other related aspects of gene expression, such as the sub-cellular localization of the corresponding gene product, can also be derived.
Similar(58)
The asymptotic confidence intervals for the parameters and hypothesis test for the parameter of interest are constructed.
On the basis of known WGD and speciation information, the structure of a phylogenetic tree for species of interest was constructed, with branch lengths derived from modal KS values from the KS plots.
A number of small combinatorial libraries of compounds reminiscent of certain designed ligands of biological interest were constructed demonstrating the potential utility of the developed methodology to chemical biology studies and the drug discovery process.
After the initial development, there is a big gap in time before applications and examples of interest were constructed.
The predictor variables of interest were constructed as follows.
Briefly, two 100 base pair sequences complementary to each mRNA of interest were constructed.
These regions of interest were constructed from assignment of a cluster of reconstructed signals around "seed" peaks from an input assignment table.
On the basis of the database information and the literature evidences, the rough PPAN, which consists of all possible static interactions among the proteins of interest, was constructed.
Double-stranded DNA templates for the RNAs of interest were constructed using PCR assembly with primers purchased from IDT Integrated DNA TechnologiesSan Diego, CA CA) (Kladwang et al., 2011b).
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