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Interest in developmental immunotoxicity testing (DIT) has generally been based on the premise that the "developing" immune system is more susceptible to external stressors than the "developed" immune system.
Philosophers of biology have shown renewed interest in developmental biology due to the potential relevance of development for understanding evolution, the theme of reductionism in genetic explanations, and via increased attention to the details of particular research programs.
The discovery of the homeobox in the 1970s [5], [6], [7] and the subsequent growth of interest in developmental genetics [8], [9], [10], [11], [12], [13], [14], [15], [16] has led to a revolution in evolutionary biology.
The regulation and evolution of sexual development have long been of central interest in developmental and evolutionary biology.
Another option would be to develop a cohort of GPs with a special interest in developmental disorders, as occurs for a wide range of physical health problems.
During the last two decades, epistemic beliefs have become a target of increased research interest in developmental and educational psychology [ 14- 16].
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We chose average monthly temperatures as we were interested in developmental plasticity during the larval stage, when individuals spend the majority of their time within a food substrate and are likely to be less exposed to the extremes of daily temperatures.
Recent discoveries that many species share the same developmental genes has renewed interest in comparative developmental biology.
We are interested in developmental-specific kinases and intriguingly, these Pt contigs also bore great resemblance to the catalytic subunit of cAMP-dependent protein kinase, known to be involved in responsiveness to mating pheromone in several fungi [ 41, 42].
A similar approach was followed for the data obtained by RT-qPCR by doing a logarithmic ratio of base 2 between the normalized quantities of the gene of interest in each developmental stage and the mean normalized quantities of the same gene in all developmental stages in analysis.
Interest in early developmental mechanisms restricted our focus to pre-metamorphic stages (∼1 day screening time), complex developmental traits like metamorphosis and organogenesis were not surveyed.
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