Sentence examples for interest housekeeping from inspiring English sources

The genes of interest, housekeeping genes and internal control are shown on Table  1.

Microarray validation ΔCt's were calculated as 2−(Ct GOI –Ct HK ) (GOI, gene of interest; HK, housekeeping gene).

Fitzgerald never became particularly domestic or showed any interest in housekeeping.

Quanti Tect primers for genes of interest GAPDH (housekeeping gene) were purchased directly from Qiagen, Germany.

Relative copy number was calculated by first determining the delta ct (gene of interest minus housekeeping gene) and then using the equation, relative copy number (RCN) = POWER 2,-delta).

PCR efficiencies for each amplicon were determined by making 10-fold serial dilutions of cDNA and then amplifying the cDNA using primers to both the gene of interest and housekeeping gene.

mRNAs were reverse-transcribed and amplified through Q-PCR using primers specific for each gene of interest; the housekeeping gene, GAPDH, was used as internal control.

Relative quantification of gene expression was calculated based on the comparative CT (threshold cycle value) method (ΔCT = CT gene of interest– CT housekeeping gene).

Nucleotide sequences of the genes of interest and housekeeping genes (Table  1) were obtained from National Center for Biotechnology Information GenBank Database, while the internal control (KanR) was supplied by Beckman Coulter Inc. (Miami, FL, USA).

We therefore believe that the gene length does not have a major influence on the evolutionary rates of the genes analyzed here.> -wrap-foot> aGOI - gene of interest; HKG - housekeeping gene Esi0130_0068 was particularly interesting due to the presence of a REJ-like domain (IPR002859).

To test this hypothesis, we have designed an array composed of 92 known human genes of toxicological interest (including seven housekeeping genes) and eight bacterial controls.