The major environmental factors considered of interest for this experiment were temperature, pH of the media, and agitation (rpm), which were optimized using Response Surface Methodology.
Such optimizations are of particular interest for trapping experiments [27, 28, 30, 33] and low-energy scattering [39, 40] applications.
This is most important for the rare, short-lived isotopes in which there is considerable interest for physics experiments.
These expressions allow the design of the beam specimen in such a way as to limit the radiation damping below a desired level in the frequency range of interest for the experiments.
Because the permanent magnets do not allow for dynamic adjustment of the transmission curve (as opposed to the electrostatic guide, see below) it was crucial to select the dimensions such that these stop bands would lie outside of the region of interest for our experiments.
Since endogenous biotin levels are low (∼10 nM in mouse), it is unlikely that substantial displacement of [F]NPB4 from avidin by endogenous biotin would occur in vivo over the time scale of interest for our experiments.
It is true that in some cases it is possible to find sequences that are fairly unique within repeat regions, which might be of interest for some experiments especially in mammalian genomes [ 17].
