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For each LT treatment, data were expressed as a ratio of gene of interest expression to 18S expression.
Relative levels of genes of interest expression were normalized to the 29 rps gene.
Thus, these activities and organizations do not provide the function of interest expression.
For nine genes of interest, expression ratios were confirmed using real-time PCR (Fig. 4).
Of interest, expression of the de novo DNA methyltransferase gene cDNMT3B was highly detected in the early embryos and PGCs.
Gene of interest expression was normalized to the reference genes PPIA and β-actin [ 46].
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Gene-of-interest expression was normalized to the geometric mean of two stably expressed reference genes (Rplp0 and Rpl10 57.
Besides optogenetics, the eDIO system represents a powerful control unit for gene-of-interest expression whenever leaky expression cannot be tolerated within stem cells or certain differentiation states.
Furthermore, a correlation between reporter expression and gene-of-interest expression is frequently lacking in these experiments.
During the period of protein-of-interest expression, l-arabinose was present in the culture to continuously enhance E. coli proteostasis network capacity (red pathway in Figure 4a).
During protein-of-interest expression, l-arabinose was kept in the cell culture to ensure that the E. coli proteostasis network capacity was constantly enhanced.
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