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The heterogeneity in staining for β-catenin and its intercellular localization may be explained by differences in the individual rates of differentiation of keratinocytes, as well as by region-specific requirements.
In both P0 and P1 cultures we could observe continuous positive signals, lining cell boundaries, confirming the expression as well as the intercellular localization of tight-junctions.
A transit peptide for the import into mitochondria was identified in the N-terminal region of PtHsp90-6, buthehe intercellular localization of PtHsp90-6 remains to be confirmed experimentally.
In addition to TEER, differentiated NPCs also regulated p-gp activity, tight junction fidelity in terms of continuous intercellular localization, and expression of various genes in a manner similar to primary astrocytes.
To address this issue of biases, we have adopted a functional referencing strategy that uses gene expression data obtained by calculating gene pairs with relevance to intercellular localization and/or molecular function.
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Previous studies suggested that the intercellular location of ESE-1 is determined by nuclear localization and nuclear export signals [ 21, 22].
Three days post NAC exposure, differentiation of NHEK is demonstrated by an increased number of intercellular junctions, basal localization of cytokeratin and apical localization of actin determined by scanning electron micrographs of cells and sub-structures and high resolution confocal fluorescence immuno micrographs of for example β-catenin, E-cadherin, actin and cytokeratins.
Reduction of flotillin-1 expression in lymphocytes reduces chemotaxis and adhesion [19], misexpression of flotillin in Drosophila embryos impairs the localization of intercellular adhesion molecules [20] and overexpression or silencing of flotillin-2 respectively increases or decreases cell spreading [21].
Immunofluorescence studies confirmed IHC and revealed a cytoplasmic expression of Nectin-4 and a clear localization at intercellular junctions between carcinoma cells (Fig. 2C, white arrow).
The functional effects of lncRNA have been widely recognized, including regulating gene expression through modulation of chromatin remodeling, controlling of gene transcription, posttranscriptional mRNA processing, protein function or localization, and intercellular signaling.
Inhibition of the vesicular trafficking processes by BFA interferes with cycling of PIN proteins and causes their accumulation in intercellular compartments abolishing polar localization and disrupting normal auxin flux with potential effects on leaf expansion.
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