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In addition, His-tagged Mcl-1 interacts robustly with recombinant VDAC1 and 3, suggesting direct protein protein interaction.
We find that Pellino1 interacts robustly with RIP1 and IRAK1, but not with TRAF6, consistent with our prediction.
Furthermore, we found that a substrate-trapping Ulp1(3)(C580S) interacts robustly with human SUMO1, SUMO2 and SUMO2 chains, making it a potentially useful tool for the analysis and purification of SUMO-modified proteins.
These experiments showed that, at least in the context of a yeast nucleus, β1-syntrophin interacts robustly with all three α-dystrobrevin SBSs, and that the leucine-to-proline mutations ablate this interaction in each case.
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The rewired proteins interacted robustly with each other, but no longer interacted with the parent proteins.
In coimmunoprecipitation assays Unc5BΔD (which causes deadhesion) interacted robustly with Rnd1, whereas Unc5BΔC (not causing deadhesion, see Figure 2A), as well as an unrelated receptor (BMPR1a) did not interact with Rnd1 (Figure 5A).
In both cases, the isolated domains interacted robustly with each other, suggesting that in the absence of constraints the human AID binds and inhibits the KD.
GST fusion protein including the 25aa peptide corresponding to the exon 36 as well as the two other similar repeat motifs identified in SCRIB C1, but not GST alone, interacted robustly with GFP tagged CH1 spectrin domain.
It thus comes as no surprise that mirror neurons are also of high interest to the field of robotics [ 19, 20], in particular humanoid robotics, as they may hold the key to solving current challenges in designing robots that can interact robustly with humans and use, for instance imitation learning to survive in unknown environments.
Furthermore, RPN2, a novel ubiquitinated target we identified for VAPB and FAF1, interacts more robustly with VAPB than with FAF1, suggesting that the interaction is unlikely to be mediated by FAF1.
Pellino2 interacts more robustly with IRAK1 than with RIP1 and TRAF6.
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