All the cleavage-based miRNA intron interactions were validated by using public degradome sequencing data.
HO-2-adiponectin interactions were validated by the two-hybrid system assay.
Currently, engineered specific Fc-FcγR interactions are validated with a static conformation derived from the crystal structure.
Some of the distal interactions were validated by siRNA knockdown experiments, including a locus involved in the regulation of multiple transcripts involved in the ER stress pathway.
Based on the analysis studies, the numerical modeling methodology for IABs, i.e., temperature loadings, backfill abutment interactions, and soil pile interactions, is validated by comparing the bridge response with the field measurements.
For additional support, a number of interesting interactions were validated by co-immunoprecipitation (Figure 4B).
Positive Y2H interactions were validated by co-immunoprecipitation (CoIP) in mammalian 293 cells as a second interaction test (double-lined red squares).
Key regulatory interactions were validated experimentally.
Inferred interactions are validated through independent evidence.
