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CK, TH, and MS hold a patent related to the content of this manuscript: 'An in vitro cell interaction culture system for testing and developing medicaments' (DE 10 10 54 20 B4).
Fifty microliters of the supernatant of C. albicans-macrophage interaction cultures were collected at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, and 24 h to determine TNF-α levels.
Since there were no interactions with culture, we analyzed the linear trends of repetition across conditions using data collapsed across cultures.
To investigate the plasma treatment effect on PCL-cell interactions, cell culture tests have also been performed.
We assumed that the differences in cell-to-cell interactions in culture and those with considered nanostructures can be explained by differences in electrochemical properties of nanomaterial substances and cells.
But instead, we go the other way -- compounding our dysfunction, and encumbering our interactions with culture with only the follies of our genomic forays.
The potential effects of enzymatic digestion, which disrupts and re-establishes the cell-cell and cell-matrix interactions in culture may account for this finding.
These structural and functional studies of crystal-cell interactions in culture indicate that COM crystals rapidly adhere to microvilli on the cell surface and are subsequently internalized.
