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Salmonella-PAM interaction was terminated 24 hours after the infection when the lysates of the cells in RLT buffer (RNeasy kit, Qiagen) were harvested.
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This interaction is terminated by hydrolysis of GTP to GDP, which is promoted by GTPase-activating proteins (GAPs) and results in switching towards the inactive conformation.
Cellular interactions were terminated after 8 h incubation by fixation with 3.5% paraformaldehyde in buffered saline at 4°C for 15 min, and mesothelial apoptosis determined by DNA fragmentation assay using the In situ Cell Death Detection Kit, Alkaline Phosphate (AP) (Boehringer Mannheim Gmbh, Mannhein, Germany) as per manufacturers instructions.
The interaction was detected using TMB Substrate-Chromogen (Dako A/S, Glostrup, Denmark), and the reaction was terminated by the addition of 1 M sulfuric acid (H2SO4).
Although the study was terminated without reaching the maximum tolerated dose, toxicity appeared potentially manageable without evidence of a significant pharmacokinetic interaction with this combination.
The investigation was terminated.
Their employment was terminated.
The contract was terminated.
Magness' contract was terminated.
In April, Hartzell was terminated.
Mr. DiCarmine was terminated this week.
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