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Consistent with previous data, under native conditions the tetrameric Kv12.2 channel immunoprecipitates with KCNE1 or KCNE3 β-subunits; and again the specificity of this interaction was assessed using the anti-Kir2.1 antibody as a negative control (Figure 5B).
Informal social interaction was assessed using self-reported surveys following the network approach.
Treatment-by-subgroup interaction was assessed using the Gail Simon test.
The statistical significance of a linear interaction was assessed using the likelihood ratio test.
Statistical interaction was assessed using the likelihood ratio test applied to the multivariable-adjusted models.
Peptide competition for the native LapD LapG interaction was assessed using an established pull-down assay (Newell et al., 2011a).
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Morphological alterations in cell-crystal interaction were assessed using scanning electron microscopy.
Effects of the two experimental factors (sperm competition risk, potential mating rate) and their interaction were assessed using general linear models, with data log-transformed as appropriate.
The significance of interactions was assessed using Bonferroni post hoc tests.
The presence of significant interactions was assessed using likelihood-ratio tests (Wald).
Inter-rater reliability for the rate of total positive interactions was assessed using the interclass correlation coefficient.
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