This interaction seems to compete with substrate binding and consequently suppress the E3 ligase activity of CHIP towards Hsp70, Hsp90, p53, HSF1, and Smad1.
However, currently available structural information suggests that bindings of the BAR domain to GTPases and to membrane lipids are incompatible, because both interactions appear to compete for the same concave surface region of the BAR dimer (Tarricone et al, 2001).
This interaction is thought to compete with translation factors to slow nascent polypeptide elongation and provide additional time for successful targeting.
In addition, Motins, including Amotl2a (Hultin et al., 2014), can bind actin and this interaction has been shown to compete with the binding of Motin to YAP (Ernkvist et al., 2006; Chan et al., 2013; Dai et al., 2013; Gaspar and Tapon, 2014).
For instance, we cannot yet reconcile the need for strong and stable interactions, believed to be required for the viability of such complexes, and the requisite of weak and transient interactions required for molecules to compete for the same target regulating their composition.
KLF4 and RELA have been reported to be functionally intertwined, as they directly interact to induce expression of selected genes [ 58], but also to compete for interaction with a co-activator, thereby inhibiting each other's activity [ 59] (Additional file 12: Figure S5).
These fusion proteins were used as possible decoys to compete with interactions between DDX3X and viral and cellular factors in cell-based assays.
The embryonic transcription machinery (abundant TBP, low levels of TBP2) is not yet fully switched to the common somatic machinery (abundant TBP, no TBP2), effectively leaving TBP and TBP2 to compete for interactions with embryonic activators and promoter elements.
To compete with the Wnt3a-V3Nter intheaction, the synthetic peptide RH3 (AWGRFLHTNCHPFLA) from V3Nter CRD was added to culture media.
Interestingly, our laboratory previously provided evidence that TP53INP1 is able to compete with p62 for interaction with LC3 thanks to its better LIR affinity (Seillier et al, 2012).
