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Here, we took advantage of BiFC to directly address the interaction status between AbdA and different candidate interacting proteins in vivo.
However, as competitive BiFC could not discriminate between AbdA- and Exd-specific interacting partners, a second experimental phase will be necessary to confirm the Hox interaction status.
Under certain conditions, signals from IR are transmitted by changes in its interaction status with its binding partners.
Everyone from businesses to governments are busy re-designing the way they work to include elements such as completing missions, competition, social interaction, status and rewarding achievement.
One such system, the split-ubiquitin membrane yeast two-hybrid system (MbYTH), is able to analyze the interaction status between two integral membrane proteins.
As a key parameter of Ab-Ag interaction, the equilibrium dissociation constant, also known as the K D value, can be used to predict the interaction status under certain conditions (Azimzadeh and Van Regenmortel, 1990; Sirin et al., 2016).
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10.7554/eLife.06034.006 Figure 2. BiFC validates the AbdA-interaction status of competitive TFs.
The designed mockup was providing multiple instances, allowing users to directly interact with the system using provided controls, offering the textual command line instruction mode for human machine interactions, status updates and tools tips.
Male is systematically significant in interaction with status for all the three variability indicators studied here.
We found a significant interaction between status and time during follow-up for abduction (Table 3).
In addition, the interaction between Status and Age was examined (Table 3).
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