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Validation with TAP protein complex purification data shows virtually no association of overhangs with their paralog's module (average interaction spoke value for overhangs is 0.06 compared to 2.6 for their "in-module" partners).
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Further to previous work, the research carried out here asked the children what type of interaction occurred (spoke, waved, no interaction, or other) which would relate to building or maintaining community connections.
Some databases represent experimentally observed complexes as interactions with more than two participants, while others expand them into binary interactions using spoke or matrix model.
Some databases represent experimentally observed complexes as interactions with more than two participants, while others expand them into binary interactions using spoke or matrix model (1).
We demonstrate that the hubs (i.e., nodes with a disproportionately high number of interactions) and spokes (i.e., nodes with fewer interactions) of the network exhibit distinct functional and etiological properties.
This is commonly done by translating non-binary CoIP associations, of a bait to the set of preys obtained by tagging it, into binary interactions using the spoke model (Bader and Hogue, 2002), where a purification is translated into a set of pairwise interactions between the bait and each of the precipitated preys.
To fairly treat the information provided by protein complexes without exaggeration, it is preferable to replace the expanded interactions, either from spoke or matrix models, with a flat list of complex members.
Sliding is converted into propagated bending, most efficiently by control of IDA activity via phosphorylation and mechanical interaction involving the radial spoke, central pair complex.
Comprehensive analysis of pairwise SYNZIP interactions revealed many interesting network patterns, such as orthogonal interaction pairs and hub-spoke motifs.
In order to integrate this type of data with those from other experimental sources, we must first derive a set of hypothetical pair-wise protein interactions using either a "spoke", or "matrix" model [ 16].
We filtered out IntAct interactions that were based on spoke expansion (connecting bait to all preys in the case of a co-complex involving more than two molecules).
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