These included MYH9, MYH10, MYL12B, ZFR and RBMX that have previously been reported to interact with USP45 in large scale DUB interaction screens (Sowa et al, 2009).
Genetic or protein interaction screens may be required to identify SUN-interacting partners and their function in plants.
Miz1 (Myc-interacting zinc-finger protein 1) is a POZ-TF that was originally identified as a c-Myc interacting protein [ 29] in yeast two-hybrid protein interaction screens.
Yachie, N. et al. Pooled-matrix protein interaction screens using barcode fusion genetics.
Two-hybrid protein-protein interaction screens were carried out for S. cerevisiae genes implicated in mRNA splicing and microtubule assembly.
Bartholdson, S. J., Crosnier, C., Bustamante, L. Y., Rayner, J. C. & Wright, G. J. Identifying novel Plasmodium falciparum erythrocyte invasion receptors using systematic extracellular protein interaction screens.
Genetic interaction screens in Caenorhabditis elegans, Drosophila melanogaster, and mammalian models are helping to improve our understanding of metazoan-specific signaling pathways.
Out of these cell lines we performed protein interaction screens by repetitive and independent immunoprecipitations followed by quantitative mass spectrometry.
Hsp90 is repeatedly identified as a component of these same signalling pathways by genetic interaction screens.
Genes required to buffer the phenotypic consequences of mutations in other loci were identified using synthetic lethal genetic interaction screens.
Tanja Kuhn, Cathrin Klumpp, Katrin Wohlbold, and Sindhu Thomas are acknowledged for technical assistance with the interaction screens.
