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Therefore, if we want to address the effects of residue interaction on the evolutionary rate, and discern them from other structural effects such as solvent exposure, the noncontact set used as reference should be restricted to avoid buried residues.
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In the case of fungus-farming ants and termites, each colony is maintained as a monoculture of a genetically single cultivar [ 42, 43], and this is thought to be a key stabilizing factor in the mutualistic interaction on an evolutionary time scale [ 44].
Moreover, connectivity depends on the evolutionary interaction between dispersal strategies on the one hand and landscape structure and configuration on the other hand, which is predicted to be species- and landscape-specific [ 5].
Further insights on the evolutionary interactions between B. siculus and B. balearicus populations might be gained by collecting bio-acoustic and mate-choice data, together with additional crossing experiments.
The latter correlation depends both on the evolutionary divergence of the interaction networks, and on experimental noise.
Why does the number of interaction partners apparently have only a slight effect on the evolutionary rate?
Obviously, more experiments are required to draw firm conclusions on the evolutionary conservation of the MBF1/TBP interaction.
Then, filters based on the evolutionary information and on the interactions analysis were applied (see Figure 4 figure supplement 1 ) resulting in a sub-network of 'hot spot' residues.
Therefore, productive epistatic interactions of the building blocks should have a strong selection impact on the evolutionary trajectories.
However, direct evidence for such an interaction is lacking, and there is an alternative explanation for the homology based on the evolutionary origins of complex I.
Similar analyses could shed light on the evolutionary consequences of other environmental perturbations and their interactions.
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