Sentence examples for interaction of this mutant from inspiring English sources

Due to poor expression and stability of the Tob W93A mutant, we were unable to test the interaction of this mutant with CNOT7 in vitro, although the mutant expressed in mammalian cells for subsequent interaction assays in vivo.

In order to further elucidate the interaction of this mutant with the enzyme's substrates, we carried out ITC binding studies.

Indeed, as expected, the interaction of this mutant variant with Rsp5 was impaired, although not abolished, relative to the wild-type control (Figure 1E), implying that the motif mediates in part the recruitment of Rsp5.

The disassembly is driven not by direct interaction of the mutant BtubB with protofilaments, but by its sequestering wild type BtubA into inactive mutant dimers.

Based on the in vitro transcription assays, we conclude that the adverse effect of the R60E mutation on trxB transcription is likely caused by either a weaker interaction of the mutant Spx with the trxB promoter, RNAP, or both.

As shown in the right panels of Figure 7a, the maximum of the interaction of the mutant B-Myb and Jnk1 already occurred 30 min after UV irradiation.

These results show that miR-122 increases the association of ribosomes with the HCV RNA in living cells and confirms the direct interaction of the mutant miR-122 with the mutant HCV RNA.

To test this possibility, interaction of these mutants with HSPA5 was analyzed in vitro by immunoprecipitation following co-expression in COS7 cells.

For example, the coactivator CREB-binding protein (CBP) binds the polyQ-expanded AR more robustly than its wild-type counterpart in a mouse model of SBMA (Sopher et al., 2004) and can be sequestered into mutant AR aggregates (McCampbell et al., 2000), suggesting that the interaction of this protein with mutant AR may be important for disease pathogenesis in vivo.

While these mutations were shown not to affect the affinity of ISW2 for nucleosomes or the interaction of its ATPase domain with the nucleosomes, DNA fingerprinting showed that the interactions of this ISW2 mutant with flanking DNA were altered and that the repositioning activity was significantly affected.

We further investigated the effects of this mutation on the binding interactions of the mutant enzyme with the substrates.