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Using FLIM-FRET and co-immunoprecipitation we additionally show extended and more intense interaction of the variant p.C284A with ASC compared with WT caspase-1.
It remains possible that the association between the variant and unexpected infant death represents a complex interaction of the variant with environmental risk factors including sleep position, bed-sharing, prenatal and postnatal tobacco smoke exposure and food security.
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The interaction of the variants with need for insulin treatment may indicate beta cell involvement in lean participants.
Alternatively, we cannot rule out the possibility that the W438 mutation affects the direct interaction of this variant Pol lambda with some other NHEJ factors.
Indeed, the partially unfolded states found in the unfolding pathways of LRM variant might be related to its tendency for mitochondrial import [ 55], also explaining the strong interaction of this variant along its folding process with Hsp70 and Hsp90 chaperones in cell-free systems [ 57].
This behavior is indicative of increased mobility (Malhas et al., 2007) and, hence, decreased stability of interaction of the A436T variant within the nuclear envelope.
Elsewhere, Church et al 68 reported an interaction of the rs9960939 variant with age on children's weight gain trajectories, and found that the A allele was associated with lower BMI in infancy but a higher BMI in later childhood.
The borderline interaction of the promoter variants with need for insulin treatment suggests beta cell function involvement in lean participants.
Our experimental approach differs from the aforementioned studies in that TTR is not pre-aggregated at the outset of the experiments–as it is likely to be the case in vivo and that we use intact cells rather than isolated membranes or synthetic bilayers to probe the interaction of the TTR variants with the cells.
Alternatively, one could treat the swarm of virus variants as an ecosystem and use the Lotka-Volterra competition (L-V) equations: (2) where r i is the maximum per capita growth rate and the coefficients α ij represent the interaction of variant j over variant i.
Elbaz et al. (97) examined potential interactions of the UCHL1 S18Y variant with smoking and pesticide use; they did not observe a main effect for the S18Y variant, and there was no evidence for an interaction with either of these environmental exposures.
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