The PamChip® arrays consist of 48 peptides encompassing the LxxLL motifs of 19 different coregulator proteins ([63], Koppen et al. 2007. Micro Array assay for Real-time analysis of Coregulator-Nuclear receptor Interaction. Manuscript submitted).
APPL1 has been shown to interact directly with Akt (49), but IRS1 phosphorylation is also increased by either APPL1 or AdipoR1 OE upstream of this putative interaction (this manuscript and Ref. 18).
Ratio trans/cis interactions The manuscript states that "A high trans/cis ratio is indicative of a poor library, since spurious ligation events will tend to be between genomic loci on different chromosomes [ref 18]".
However, we believe it is worth documenting the interaction in the manuscript, given that the genetic experiments are now stronger and the genetic and electrophysiology experiments are consistent in indicating repression of RYE by SSS.
JKW: patient interaction and diagnosis, drafting of manuscript, final approval of manuscript, PA: patient interaction and diagnosis, final approval of manuscript, DYSC: patient diagnosis, final approval of manuscript, NLT: patient interaction and diagnosis, final approval of manuscript.
OW: Contributed to the study of protein-protein interaction and critically reviewed manuscript.
More specifically, the affinity of the Rh5-basigin interaction reported in your manuscript is significantly stronger to that reported in the original Crosnier et al. paper.
The description for this result has been added in the revised manuscript (subheading "Interaction between IREF-2 and viral RNA polymerases").
We would prefer to omit the AP-4 Y2H interaction data from our manuscript due to these technical issues, but we have included the results below for the benefit of the reviewers.
TH: patient interaction and diagnosis, drafting of manuscript, final approval of manuscript.
IKJ performed the comparisons between evolutionary rates and the number of protein-protein interactions and drafted the manuscript.
