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By this method, interaction evidence from model organisms was used when orthologs of interacting proteins could be clearly identified in M. tuberculosis.
For example, rule GO_AR:0000018 states that every physical interaction evidence (GO IPI/ECO:0000021) is accompanied by a supporting interacting protein or other molecule.
It acts as a meta-database which maps all interaction evidence onto a common set of genomes and proteins by integrating information from numerous sources, including experimental repositories, computational prediction methods and public text collections.
In order to complement previously published protein interactions, experimental interaction evidence was collected from curated interaction databases.
The website contains rich functionality for graphical and tabular analysis and allows components of interaction evidence to be aligned and studied both within and across species.
C. elegans protein-protein interactions (PPIs) based on experimental interaction evidence from two databases were used: the C. elegans Molecular INeraction database (MINT) database[38] (July 2009) with 7,353 PPIs and the IntAct PPI database[39] 10,445 (September 2009).
Similar(25)
Expression of DN-H-Ras DN-H-Ras DN-H-Ras NGF-induconstruct-Rac1 interaction, evidenced blockednhibition in the amount of Tiam1 co-precinteractionth Rac1 (Figurevidenced
Thus, the interaction evidenced by co-precipitation experiments shown in Figure 4 appears to be operational in HEK293 cells, allowing BCR molecules to drag the TLR4 to the cell surface.
The interactomes are built using MImerge to integrate protein interaction evidences from IMEx resources.
We combined all interaction evidences into an integrated network for each species and identified functional modules from these integrated networks.
A number of services have been built based on MIscore and MImerge, which allow users, with or without technical skills to merge and score interaction evidences.
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