Fifty microliters of the supernatant of C. albicans-macrophage interaction cultures were collected at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, and 24 h to determine TNF-α levels.
Specific parameters that are discussed include media supplements, oocyte granulosa interactions, culture atmosphere requirements, and maintenance of three-dimensional architecture.
To examine cellular growth, signalling processes and cell-cell interactions, culture systems have been developed combining the two major cell types of skin, keratinocytes and fibroblasts.
Semiconfluent monolayers were infected with overnight LB broth cultures of tested isolates diluted in interaction medium (culture medium with 5% FCS) to a multiplicity of infection (number of bacteria per cell) ∼100 1, and cocultured for 4 h (37°C, 5% CO2).
The answer has to do in part with the interaction between culture and natural resources.
Network analysis focuses on the interaction of culture, human agency, and social structure.
There is also a touch of the old Eagleton when he deploys Thomas Hardy's Jude the Obscure to explore the interaction of culture and materialism.
A better portrait of the flickering interaction between culture and reality in the belly of the beast is "Adaptation," the most recent film by Spike Jonze and Charlie Kaufman.
"There is a lot of faddishness with beards, they are on the way back and it's interesting to look at that interaction with culture," he said.
