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In our protein-protein interaction assays both AtYAF9A and AtYAF9B interacted with AtSWC4 and AtEAF1.
DNMT3L interacts specifically with unmethylated histone H3K4 in in vitro interaction assays (34– 34).
Together, these results suggested that our vectors are adequate for protein expression and protein-protein interaction assays in rice protoplasts.
Also, using output data of DNA protein interaction assays, we found that 42 of all predicted genes are targets of SOX2, NANOG and POU5F1.
Using standard biochemical protein-protein interaction assays, we found that Wise can also bind to Lrp4.
We used in vitro DNA protein interaction assays designed to identify mDNA target sequences for dSETDB1.
We therefore expressed and purified recombinant HrpB2XAC to perform in vitro interaction assays with HrcUXAC_207 264 and HrcUXAC_207 357 AAAH).
Ubc13 was preincubated with the test compounds at varying concentrations, and interaction assays run in a Biacore T100 instrument.
The four WW-domains of Nedd4 were therefore also tested for their interaction with SGK using GST interaction assays (see Fig. 1E).
However, in the interaction assays with normal neutrophils the mutant strains did not show an increased susceptibility to the phagocytes (data not shown).
(H ) Yeast two-hybrid interaction assays between FAMA and RBR.
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