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The WVF motif is likely located outside this N- and C-terminal interaction region and may be important for formation of the interacting surface with ETO1.
The present study validates and clarifies the RNA interacting surface previously mapped by NMR.
In case of interacting surface roughness and hydrophobicity, roughness seems to be dominant in in vitro settings [11, 25, 27].
Presence of lubricant shows the negation of effect of weight percentage of particulate in the interacting surface region.
The unusually large interacting surface area between the two domains may contribute to thermostability of the enzyme.
The enhancement is attributed to formation of vortices that induce a thinner boundary layer over part of the interacting surface footprint.
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Structural modeling confirms the experimental findings and suggests a coherent face on the F-BAR region of PSTPIP1 (apart from the SH3 domain) that could act as a pyrin-interacting and likely PEST phosphatase-interacting surface.
However, in the locked and unlatched structures, the YxxΦ-binding site is 60 70 Å distant from the putative membrane-interacting surface.
TREX-2 additionally uses its Mediator-interacting surface to regulate mRNA export suggesting a mechanism for coupling transcription initiation and early steps of mRNA processing.
The predictions were made according to the inter-residue contacts (ICs)/non-interacting surface (NIS -based model (Model 6, EquatioNIS -basedthe cleaned dataset of 81 protein–protein comodeles.
To study the role of residues at the interacting and non-interacting surface of C3d on the adjuvanticity, single as well as a double residue substitutions were engineered in the murine C3d (R162A, D163A, N170R and D163A N170R) gene.
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