Overall these findings point to distinct Ca2+ sensitivities that are determined by two probably interacting sites within the IML2 of UCP3.
To identify regions that are frequently associated with the bait region other than random collision, we applied statistical models to analyze interacting sites within each chromosome.
In contrast, allosteric modulation between two simultaneously bound and interacting sites, either within a receptor monomer or across a receptor dimer/oligomer, can alter ligand dissociation (6).
All the interacting sites within ±1 Mb range of a positively interacting site were clustered as an enriched interacting domain.
For sonication-based data, each interacting site was assigned a z-score based on the nearby interactions observed within ±1 Mb distance range (see Methods for details).
Although this motif has ligand attributes, it is not within the previously characterized interacting sites.
Although the in vivo-selected homing motif CGRRAGGSC had certain ligand-specific attributes [1], [2], the corresponding location within the native cytokine is not in an established interacting site between IL-11 and IL-11Rα [17]–[28].
The cavities within pseudo wild-type and L121A lysozymes each bind only a single atom of noble gas, while the cavities within mutants L133A and F153A have two independent binding sites, and the L99A cavity has three interacting sites.
Identification of coevolved residues can provide insight into pairs of amino acid sites within a protein whose evolution is linked by structural, functional, or interacting constraints.
This might be a direct effect of ligand binding (e.g. steric blocking) if Cys is located close to the drug-binding site, and the differences observed for the various drugs could reflect the fact that they interact with different sub-sites within a large flexible substrate-binding pocket.
Up-regulated ATF6, ATF4 or XBP1S expression induces apoptosis by interacting with binding sites within the promoter of the CHOP gene.
