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Much of the work discussed here has been performed either in oocyte or reticulocyte lysates, where other interacting proteins may be present.
The first reviewer felt that the expression of the interacting proteins may be in excess and this would result in a skewed kinetic landscape.
Other studies have focused on using literature curated databases of molecular pathway interactions, thus taking the alternative view that consistency and trends in mRNA expression levels of interacting proteins may be used to infer pathway activity [ 6- 8].
The complementarity of GO terms (i.e. the tendency of a pair of GO terms to be associated with each of two interacting proteins) may be computed from the GO annotations of protein pairs in the overall protein-protein interaction network [ 15].
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Since such additional Ii-interacting proteins may be contained in all Ii-complexes that are obtained by chemical crosslinking, it must be assumed that the crosslinked high molecular weight material that is detected by immunoblotting for DR and for Ii at the top of the gel (Fig. 4A, lower panels) does not solely consist of DR and Ii but contains other cellular proteins.
However such membrane-interacting proteins cannot be detected by current bioinformatics tools and consequently the number of membrane-interacting proteins may be much larger in cells than currently recognized.
The availability of a large number of such known interacting sequence pairs additionally provides a solid statistical basis for developing computational methods to deduce the rules of SK/RR interaction, so that the remaining half of the interacting TCS proteins may be understood.
For instance, CAND1 binding to cullin proteins may be regulated by posttranslational modifications in either of the proteins or by other interacting proteins.
To test whether other LET-418 interacting proteins may also be involved in the control of pgl-1 expression, we fed L1 larvae with dsRNA corresponding to all genes encoding NuRD complex components.
The biological activity and expression of ErbB2 and ErbB3 are regulated by a host of interacting proteins that may be potential targets for development of new therapies for treatment of hormone resistance.
The nuclear matrix attachment proteins SAFA/HNRNPU and SAFB, which were also identified as NKX3.1 interacting proteins, may also participate in this process.
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