Most importantly, glutathione S-transferase pull-down assays demonstrated the MMP-2 as a new PAK4-interacting protein which binds to PAK4 kinase domain.
Contradicting reports suggest that ROS solely acts to upregulate NLRP3 and pro-IL-1 β expression in an NF κB-dependent manner [ 235], and other studies demonstrated how ROS can be sensed by a complex of Thioredoxin (TXN and TXNN interacting protein (TXNIP) which binds to NLPR in conditions of oxidative stress [ 236].
Co-expressed genes frequently code for interacting proteins, which in turn leads to new insights into protein function(s).
Single-stranded DNA coated by the single-strand DNA-binding protein RPA is bound directly by ATRIP (ATR-interacting protein), which enables the association of the ATRIP ATR complex at stalled forks (Zou and Elledge 2003).
Among these interacting proteins, PI3K which binds to the phosphorylated tyrosine 740/751 residues (739/750 in the mouse) of PDGFRβ through its p85 regulatory subunit [9], is particularly important for regulating actin organization [10], [11], cell growth and migration [12].
The CTNNBIP1 gene encodes for the β-catenin-interacting protein 1, which binds β-catenin to prevent interaction with TCF, thereby inhibiting Wnt signalling (Tago et al, 2000; Yemelyanova et al, 2011).
First, a protein that forms homodimers (a self-interacting protein) may undergo duplication, leading to two identical self-interacting proteins which also interact with each other.
The second identified component of A-type potassium currents from our microarray screens is Kcnip4, a member of the calcium-binding protein family of KChIPs (Kv channel interacting proteins), all of which bind to cytoplasmic Kv4 channel N-termini.
Other mechanisms for transport of APP by kinesin-1 have been described, including direct attachment of APP to KLCs, or indirect attachment to KLCs via adaptor proteins such as JIPs (C-Jun N-terminal kinase interacting proteins) and PAT1 which bind to both KLC and APP (14, 19, 41– 41).
Chou et al. used the yeast two-hybrid method to identify a novel GSK3 β interacting protein, GSKIP, which can bind to GSK3 β and inhibit its kinase activity.
TNF stimulation results in the recruitment of TNF receptor-1-associated death domain (TRADD) protein and of receptor interacting protein 1 (RIP1), which function as adaptor proteins for the E3 ubiquitin ligases TNF receptor-associated factor (TRAF) 2 and TRAF5, which in turn bind the E3 ubiquitin ligases cellular inhibitor of apoptosis (cIAP) 1 and cIAP2.
