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In this work, three key positions in the hydrophobic core were randomized in a VPE phage displayed library (Figure 1). Figure 1 VPE-VPK represented as (a) a helical wheel diagram and (b) a ribbon diagram, with randomized positions in yellow, and the directly interacting position in green.
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Interacting positions were defined as being less than 4 Å apart because this is the upper limit for weak interactions (Martin et al. 1997).
In the first, some variants are fixed by positive selection to compensate deleterious mutations in other epistatically interacting positions.
This strategy was adapted to a multi-round framework of experiments where the data generated in the present round is used to design experiments for the next round to improve the generated library, as well as the estimation of the interacting positions.
The strong tendency of correlated ionic interactions to be spatially coupled has been observed previously in the study involving the double replacements of interacting positions (DRIP) [5].
The chief interacting positions between sheet and the helical segment and the pattern of key stabilizing hydrophobic interactions are conserved throughout the fold, supporting its monophyletic origin.
To avoid large, overlapping confidence intervals, interacting QTL positions were selected when the genome regions involved were larger than 30 cM.
This would then suggest that small hydrophobic amino acids, and then glycine, might similarly cause pauses by interacting with positions one or three amino acids further out in the exit tunnel.
Finally, given the time dependency in the output of an onset detection algorithm, when the user interacts at position t int of the O t), all information located at time frames t
To test Hypothesis 5, we interact positioning on the cultural dimensions with the presence of a regionally based ethnic group.
In one active site, A61 fluctuates toward a solvent-exposed position behind H121, whereas in the second active site, A61 remains in the starting position, interacting with F55 and F128.
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