DOI: http://dx.doi.org/10.7554/eLife.00220.011 Next, we performed a qualitative interaction assay to determine whether agrin can interact independently with the APP ectodomain.
On the Earth, gravity- and radiation-driven geomorphic systems interact independently, so that their two types of activity can mingle under conditions of periodic random dominance.
We refer to this scenario as open society, in which any pair of users (mean field approach) can interact independently of how similar are they to each other.
An optimal linker length between the charged and hydrophobic moieties was also observed to enhance retention, balancing the steric accessibility of the hydrophobic moiety with its ability to interact independently of the charged group.
The former simultaneous interaction of acid and base can be further categorized into two parts; a part in which both acid and base interact with the same substrate molecule in concerted manner (concerted mechanism), and a part in which acid and base interact independently with different molecules (go-together mechanism).
This suggests that Tim and Plk1 polypeptides can interact independently of other cellular proteins.
As expected, the EVH1 domain of VASP interacted independently of Fyn with ADAP-C [32].
In endoderm formation, β-catenin interacts with Sox17 independently of TCF-4 to activate endoderm genes [68] and in breast cancer, β-catenin and Sox2 interact independently of TCF-4 to activate Cyclin D1 [69].
FOXP3-ΔN contains a leucine-zipper domain reported to mediate homotypic interactions, that is mutated in a subset of IPEX patients [9], [16], suggesting that the A1 and A2 sequences might interact independently with FOXP3 forkhead domains within a FOXP3 multimer.
Other examples of multivalent NLS domains include the adenoviral protein pVII, which contains three NLS regions that bind to importin α, importin β1, importin β2 and importin β7 [46], and HPV16 E6 oncoprotein, which interacts independently with importin α, importin β1 and importin β2, in both classical and non-classical nuclear import pathways [69].
