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At the amino-terminus (aa 1 54), a GAT/TOM1 interacting domain is also described [3].

Recently a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was also found negatively related to butanol tolerance [ 21].

Interestingly, the Ra domain is also able to weakly interact (Figure 2D, lane 5).

MBD4, possessing a DNA glycosylase domain, is also involved in BER following deamination events and is able to interact with mismatch repair proteins [ 49, 50].

Recently, Nck has been shown to interact with SAP via its second SH3 domain, but affinity was greatly enhanced when the third SH3 domain was also present.

The activity of Gla domains is also Ca2+-dependent, as these ions both stabilize the folded structure of the domain and also interact with PtdSer (Bandyopadhyay, 2008; Huang et al., 2009).

Composite domains were also observed among polymerases.

Overlapping interacting domains were further merged together.

The amino terminus of spartin encodes a microtubule interacting and trafficking (MIT) domain that is also found in trafficking proteins involved in protein degradation through the endosomal/lysosomal system.

The association between BAF and the LEM domain proteins is also conserved between species, as Drosophila BAF interacts with emerin and Bocksbeutel, a LEM domain protein expressed as two isoforms, one lacking the transmembrane domain [33] (Fig. 2).

A ligand binding domain that interacts with estradiol is also located in the AF-2 region [ 10, 11].