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Interestingly, SMRT is recruited to PPARγ via its C-terminal interacting domain, and mutation of the proximal interacting domain does not interfere with recruitment via PPARγ.
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Interestingly enough GAF domain does not interact with the GGDEF domain, but interactions were found between the linker 1 residue 1 residue
In contrast to RMF from E. coli, the HPFlong C-terminal domain does not interact with the SD-aSD sequence but with protein uS2 at the dimer interface.
The Pfa1 C-terminal domain lacking the OB-fold domain does not interact with Prp43 [ 60].
In the structure of active Src the SH2 domain does not interact with the kinase domain and has no influence on the active state of this kinase.
This shows the Kirrel3 extracellular domain does not interact with Kirrel1 or Kirrel2 and the FLAG tag does not interfere with homophilic binding.
In a ternary complex, the TAZ2 domain does not interact directly with pRb, being engaged in the complex formation via its binding to E1A.
The study with this antibody revealed the importance of the Sp for effective aggrecanolytic activity of ADAMTS-5 and that this domain does not interact with sulfated glycosaminoglycans (GAGs) but with the protein moiety of the proteoglycan.
However, the CrkL-SH2 domain does not interact with Bcr-Abl, either by pull-down experiments (Additional file 3C) or in Two-Hybrid analysis (Additional file 4: Method 1 and Additional file 3D), indicating that the interaction between CrkL and Bcr-Abl, involving the Abl-SH3 domain, is not direct.
The LIM domain does not directly interact with DNA, but acts as an adaptor molecule for transcription factors facilitating assembly of large transcriptional complexes (Breen et al, 1998; Jurata et al, 1998; Sugihara et al, 1998).
In spite of its name, the Ste50 RA domain does not seem to interact with Ras proteins.
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