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As shown in Figure 1e, while SPOP-WT and SPOP-ΔBTB interacted efficiently with ER α, the interaction between SPOP-ΔMATH and ER α was totally abolished.
All three fragments interacted efficiently with GFP-lamin A (Fig. 2D).
Moreover, recombinant GST-Esrrb also interacted efficiently with Mediator and RNA pol2.
The predominant membrane localization of FtsY in bacteria also mitigates an otherwise crucial obstacle to the bacterial SRP cycle: if SRP and FtsY interacted efficiently with each other in the cytosol, they would probably undergo futile cycles of GTP hydrolysis.
We found that both wild-type HIV-1 (CH106) and SIVcpz (EK505) Vpus that block NF-κB activation as well as a group N (YBF30) Vpu that increases its activation interacted efficiently with β-TrCP.
We expressed and purified mCD4 and Vpu from E. coli, confirmed that purified Vpu can be phosphorylated efficiently by purified Casein Kinase 2 (CK2; data not shown), and that the cytosolic domain interacted efficiently with βTrCP in a phosphorylation-dependent manner.
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Since the MglA mutant proteins still interact efficiently with MglB as shown in vitro, the interaction between MglA and MglB that regulates reversal frequency and localization apparently depend on MglB GAP activity.
The chloroquine conjugated gold nanoparticles (GNP Chl) were found to interact efficiently with BSA.
Deshielded fluorine containing fragments, suitable for interacting efficiently with the amphiphatic α helix secondary structural motif and hydrophobic pockets on proteins, are discussed.
Capsular staining revealed that carbapenem resistant E. coli has a capsule (Supplementary Fig. 6), since most capsular polysaccharides are negatively charged, electrostatic repulsion might have prevented SDS from interacting efficiently with the bacterium.
Using different biophysical techniques and docking simulation analysis, we indicated that these complexes were also capable to interact efficiently with DNA via a non-intercalative mechanism.
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