In particular, arginine 12 and lysine 24 interact strongly and with high specificity with the phosphate group of the DPPG lipids, stabilizing the position, the orientation, and the secondary structure of the peptide in the monolayer.
Additional in vitro binding and coimmunoprecipitation assays confirmed that FLRT3 and Unc5D ectodomains interact in solution with high specificity (Figure 1B).
The structural plasticity allows them to interact with multiple targets with high specificity and yet low binding affinity, which is crucial for the rapid response to signaling events (79, 80).
Designer cellulosomes are artificially constructed complexes of enzymes, generally equipped with a dockerin module that interacts with cohesin module with high specificity and affinity and degrades the polymeric cellulose efficiently (Vazana et al. 2012).
A review of the literature demonstrates that Hsc70, in general, preferentially interacts with hydrophobic peptides, with high specificity for basic or positively charged residues, particularly lysine and arginine.
As cell signaling is a dynamic process, where proteins at low concentrations must often interact and dissociate rapidly and yet bind with high specificity, highly evolved protein-protein interactions are expected, to facilitate efficient recognition.
With their unique sizes in the range of viruses and proteins, nanomaterials can interact with biological systems at the molecular level with high specificity [5].
The expectation is that single target molecules with high specificity might not have access to complex interacting neural pathways, and that moderate potency could engender fewer off-target side effects.
Bacterial endotoxins (lipopolysaccharides (LPS)) are strong elicitors of the human immune system by interacting with serum and membrane proteins such as lipopolysaccharide-binding protein (LBP) and CD14 with high specificity.
A disjunction of such motifs often can represent the entire superfamily with high specificity and sensitivity.
Importantly, LRPAP1 can be used for a novel therapeutic approach that targets MCL with LRPAP1-reactive BCRs with high specificity.
