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Surprisingly, HupBMtb interacted proficiently with AT rich DNA forming two gel-retarded complexes (C1, C2; Figure 5A marked by arrowheads) where as its interaction with GC rich DNA was negligible.
Since the average GC content of the Mtb H37Rv genome works out to around 65%, we wondered whether the CTR of HupBMtb would endow it with the ability to interact more proficiently with GC rather than AT rich DNA.
On the other hand HupBMtbN while retaining its ability to interact more proficiently with AT rich DNA, forming a single gel retarded complex (Figure 5B, lanes 9 14), displayed weak interaction with GC rich DNA (Figure 5B, lanes 2 7).
We propose that the Cterm acts as a chaperone by direct physical contact with the mutated mt-tRNA in vivo, helping it to maintain the conformation suitable to proficiently interact with its cognate aminoacyl-tRNA synthetase or other molecular partners.
An interesting finding was that HupBMtb interacted much more proficiently with substantially A T rich than with G C rich DNA.
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Together with the ladder-like phylogenetic trees of EV-A7120, these factors suggest an evolutionary scenario of a proficiently host-adapted virus engaged in intense immune evasion mediated at a small number of virus particle surface sites that interact directly with the host immune system.
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