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pGBKT7-P53 (Murine p53 fused to GAL4 DNA-BD) and pGADT7-T Antigen (SV40 large T-antigen fused to GAL 4 DNA-AD) were used as positive control in these experiments, as p53 and T Antigen have been shown to interact in a yeast 2 hybrid assay earlier [33].
Interestingly, Tjp1a and Cx39.4 and Cx41.8 can interact in a yeast 2-hybrid assay.
Based on these observations, along with the finding that Mei-P22 and Vilya interact in a yeast two-hybrid assay, we propose that Mei-P22 acts upstream of Vilya, and recruits Vilya and Mei-W68, which catalyzes DSBs.
In agreement with these studies, we found that by combining AN3 OE with GRF5 OE, shown to interact in a yeast two-hybrid assay (Horiguchi et al., 2005), the leaf size is increased more than expected.
It was previously demonstrated that WRKY70, together with the HASTY TF, and Zat7 actually interact in a yeast two-hybrid assay through the EAR-domain of Zat7 (Ciftci-Yilmaz et al. 2007).
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Previously we reported that AES and LRP6-ICD interacted in a yeast 2 hybrid assay [39] but the interaction was not confirmed in situ.
To confirm bioinformatic predictions, 15 cis-sequences were used for the isolation of interacting TFs in a yeast one-hybrid screening.
We have shown that Irgm1 can interact with itself in a yeast 2-hybrid system, and self-interaction is abolished by destroying the nucleotide-binding motif (S90N) [8].
TFIIbeta has been shown to interact with Ubx in a yeast two-hybrid assay [ 41], while BIP2 has been shown to interact with Antp [ 47].
It was reported that tankyrase 2 can interact with IRAP in a yeast two hybrid system [17]; however, the involvement of tankyrase 2 in regulation of GSV trafficking has not been defined.
Fab1p does not interact with Fig4p in a yeast two-hybrid test.
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