Upon serum deprivation for 24 h, the medium was then removed and cells were washed intensively in PBS and culture medium followed by stimulation with fresh serum-free medium (SFM) or SMK-containing medium for 10 min. The cell medium was then collected for analysis.
The fine fraction <20 μm was obtained by shaking a horizontal shaker at the beginning of the procedure and the fine fraction <20 μm gained after re-sieving the ultrasonically dispersed soil was combined and obtained by centrifugation by 4000 g for 18 min. The supernatant was removed and the settled sample was washed intensively with deionised H2O.
Liver regeneration has been studied intensively since the introduction of a rodent partial hepatectomy (PH) model, in which 2/3 of the liver mass is removed.
With intensively grown adult cucumber plants, however, Marcelis (1991) observed no reduction in leaf photosynthesis until 16 d after the fruit was removed, whereas thereafter photosynthesis and dry matter growth fell sharply.
Corn stover from two replicate beakers treated under similar conditions was recovered, combined, and washed intensively with deionized water to remove soluble phenolics and other degradation products.
After a certain exposure time, the onion peels were intensively rinsed in deionized water to remove the NPs deposited onto the epidermis surface and unincorporated inside the epidermal cells.
Convective heat transfer of conventional heat transfer fluids inside micro/minichannel heat exchanger has been intensively studied due to its capability to remove high flux [27, 31, 61 67].
While the social systems linked to trepang fishing and trade have intensively been researched, the ecological effects of removing a large number of bioturbating organisms from shallow tropical ecosystems for the most part are still unknown [56].
Two-day old spores of A. tumefaciens grown on yeast extract broth were harvested by centrifugation for preparing their CWE. A. brassicae mycelium, propagated on PD broth for 14 days, was filtered through four layers of sterilised nylon membrane and intensively washed seven times with sterile H2O to remove the medium and spores.
For the antibody-binding experiments, the surface was first functionalized with the MPs as described above, and then the chip was exposed to 5 nm of fluorescently labeled anti-BR for 30 min. The chip was incubated and washed intensively with PPB-T20 for 10 min in order to remove nonspecifically adsorbed molecules.
