Sentence examples for intensive washing from inspiring English sources

Between each step intensive washing with TBS containing 0.025% Tween 20 was performed.

Following intensive washing with PBS containing 0.05% Triton X-100 (PBS-T), plates were incubated with a peroxidase-labeled monoclonal anti-HA antibody (12CA5; 1 mg/mL PBS-T).

Following intensive washing, the incubation with the secondary antibody Cy3-conjugated donkey anti-rabbit IgG (1∶1000; Jackson ImmunoResearch, West Grove, PA, USA) in TBS containing 0.3% Triton X-100 and 5% FCS for 2 hours at room temperature was performed.

Briefly, parasites were isolated by lysing the red blood cell in saponin containing buffer (7 mM K2HPO4, 1 mM NaH2PO4, 11 mM NaHCO3, 58 mM KCl, 56 mM NaCl, 1 mM MgCl2, 14 mM glucose, 0.02 mM saponin, pH 7.5) followed by intensive washing.

Nonspecifically absorbed phages were removed by intensive washing.

Non-specifically absorbed phages were removed by intensive washing.

On the several days I used them they left scum on the glasses and food on the plates, even after an intensive wash.

After intensive wash, the remaining cells were incubated for 24 h in the presence of 50 ng/ml KGF.

After an intensive wash, the filters were developed with an enhanced chemiluminescence (ECL) kit, following the procedure described by the manufacturer (Amersham, Buckinghamshire, UK).

All of the antibodies were diluted with 1% bovine serum albumin (BSA) in PBS, and the antibody incubations described above were preceded by intensive washes in PBS.

Furthermore, these enzymes may be useful for processing ionic liquid-treated biomass without the need of intensive washes to dilute ionic liquid residues, thus helping to reduce the use of water after the ionic liquid treatment.