In CO, the OHCs and DCs displayed more intensive staining for A20 (m), the OPCs and DCs exhibited more intensive staining for RNF11 (o).
In the cochleae exposed to 0.4 % AgNPs, the SLFs showed more intensive staining that was independent of the cochlear turn, while the SIMCs, SBCs, and capillary endothelial cells (CaECs) demonstrated more intensive staining in the first turn (a c).
In the cochleae exposed to 0.4 % AgNPs, the outer hair cells and Deiters' cells of Corti's organ showed more intensive staining for TLR2 (Additional file 2: Figure S2E).
The inner hair cells, pillar cells, and Deiters' cells displayed more intensive staining for TLR4, but the outer hair cells did not (Fig. 2g).
However, the strial intermediate cells, strial basal cells, spiral ligament fibrocytes, spiral ganglion cells, and hair cells, pillar cells, and Deiters' cells of Corti's organ showed intensive staining for Erk1/2 (Additional file 8: Figure S7B, S7D, and S7F).
In the cochleae exposed to dH2O, the strial basal cells, spiral ligament fibrocytes, root cells, spiral ganglion cells, pillar cells of the organ of Corti, and capillary endothelial cells in the modiolus showed intensive staining for TLR2.
In the cochleae exposed to dH2O, the spiral ganglion cells (SGCs) showed intensive staining for IL-10 (F), while the pillar cells (PCs) of Corti's organ (CO) demonstrated mild staining for IL-10 (J).
The strial basal cells, spiral ganglion cells, and inner pillar cells of Corti's organ exhibited intensive staining for RNF11, while the spiral ligament fibrocytes, hair cells, and outer pillar cells displayed mild staining for RNF11 (Fig. 4h, l, p).
Unexpectedly, the strial basal cells, spiral ligament fibrocytes, spiral ganglion cells, and the hair cells, pillar cells, and Deiters' cells of Corti's organ showed intensive staining for MCP2 (Additional file 3: Figure S3B, S3D, and S3F).
In the cochleae exposed to 0.4 % AgNPs, the SBCs and SLFs demonstrated more intensive staining for A20 and RNF11 that was independent of the cochlear turn (a c, e g).
The SBCs, SGCs, and inner pillar cells (IPCs) of CO exhibited intensive staining for RNF11, while the SLFs, hair cells (HCs), and outer pillar cells (OPCs) displayed mild staining for RNF11 (h, l, p).
