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This leads to intensive shaking of the rocks around the hypocenter, which in turn generates elastic waves.
In those conditions, neurons do not survive; microglial cells were collected by intensive shaking of culture flasks for 1 h at 37°C at 250 rpm in orbital shaker.
A possible solution could be fixing the gel >3h or overnight and intensive shaking to remove SDS-carrier ampholyte complexes.
After a new incubation of 6 h, cultures were killed by adding 1 mL of chloroform, followed by intensive shaking.
After intensive shaking and after centrifugation, the supernatant was transferred to a clean tube and the solvent was evaporated at 40 °C under a gentle stream of nitrogen.
Briefly, the cells were lysed with CellTiterGlo® reagent and after intensive shaking the lysates were transferred to white 96-well plates (Greiner Bio One) and measured on a Victor 2 reader under standard luminescence settings.
Similar(54)
Strains were grown on a BIOSCREEN C (distributed by Thermo Electron) in triplicate in a total assay volume of 300 μL at 30°C with intensive on/off shaking [ 47].
Two weeks later, microglia were obtained by intensive washing and shaking the culture (250 rpm) for 1 h at 37 °C.
Yet Weiland's anything but discouraged, betting that his labor-intensive strategy of shaking hands and chatting with every possible voter will pay dividends in November.
Once again, images of squirming infants struggling to hold on to their first minutes of life and stories of newborns shaking from withdrawal in neonatal intensive care units have reclaimed media attention.
Emulsions are formed traditionally by homogenization (shaking, stirring or some other kinds of intensive dynamic and/or static mixing processes).
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