A more intensive labelling, using 20 confocal scans rather than 6 also failed to stimulate phospho-H2AX.
However, one O. flexuosa nodule containing a young male showed intensive labeling of the testes similar to that seen in male A. viteae (Fig. 3F, H).
Consistent with these results fluorescence microscopy of wild-type parasites labelled with NBD-lipids revealed an intensive labelling of intracellular membranes with NBD-PC and -PE but not with NBD-PS and -SM (Fig. 2D).
Intensive labeling of butyrophilin occurs on both the P face of the monolayer and the E face of the bilayer (Fig. 11, 12).
Using the E- and P-selectin fusion proteins, an intensive labelling of all HT 29 cells was noted when they had been grown in vitro, whereas only labelling of some HT 29 tumour cells in primary tumours grown in both strains of mice was noted, and in particular when the tumour cells had grown adjacent to blood vessels.
The nafion/Cu3(PO4)2/SPCEs have great promise for estimating the freshness of fish meat without expensive separation equipment and labor-intensive labeling procedures.
Whole blood or phosphate buffer saline spiked with low numbers of pancreatic cancer cells were successfully detected by specially designed microfluidic device incorporating an AAO RIfS sensor, without labour intensive fluorescence labelling and/or pre-enhancement process.
Three types of staining patterns were observed: (i) homogeneous cytosolic as seen with full length B- and C-RAF (see 142 606 construct in Figure 1C), (ii) a punctate pattern (see 88 606 construct in Figure 1C), and (iii) an intermediate pattern, with intensive homogeneous labeling of plasma membranes (see Figure 1C, 1 149 construct as an example).
Applying immunohistochemistry for detection of BtRJPL-ir in HGs, an intensive cytosolic labeling of HG secretory cells in both bumblebees and honeybees was observed (Fig. 2A).
In video semantic classification, most of the learning-based approaches require a large training set to achieve good generalization capacity, in which large amounts of labor-intensive manual labeling are ineluctable.
