In order to minimize the very time-consuming and cost-intensive isolation of primary brain capillary endothelial cells (pBCEC) brain capillary endothelium cell lines, as the bEnd3, were used for cellular uptake and subcellular distribution studies.
For this reason the use of cultured mammalian cells to produce recombinant proteins (except at very small scale) usually requires labour-intensive isolation of rare, clonal cell lines that stably express the transgene at high levels.
The clinical picture of typhoid fever is nonspecific; confirmed diagnosis through blood or bone-marrow culture requires expensive and labor-intensive isolation and identification of the organism, which may take up to seven days.
The use of the heat-treatment method for template preparation provides a good alternative to the expensive and labor intensive DNA isolation methods that might not always be possible in field settings.
Minimizing the number of negative samples is expedient from a laboratory perspective, particularly when labor-intensive virus isolation techniques are being used.
Moreover, the nested PCR-EIA is easy, rapid, and less labour-intensive than isolation and non-viable Chlamydiaceae can be detected, due to the relative high stability of DNA.
Access to microbial diversity traditionally focused on intensive sampling and isolation using general methods from a wide range of geographical locations and habitats, with recurrent isolation and screening of the predominant species and a low probability of isolating novel compounds.
A bacterial isolate, tentatively nominated as S33 and previously (Embaby et al. 2014a) isolated from agricultural soil contaminated with pear fire blight pathogen through an intensive program for isolation of antimicrobial activity producing bacteria, was used in this study as a siderophore producer.
Assumptions are made for the effectiveness of two approaches that combined several case-based public health measures (combination of case-based measures; CCM): Intensive measures, including isolation and therapy of cases, contact tracing, quarantine and post-exposure prophylaxis of selected contacts in- and outside of the household (CCM1).
Despite the intensive studies on isolation and characterization of B. anthracis phages, many questions still remain unanswered.
Pneumothorax often complicates the management of mechanically ventilated severe acute respiratory syndrome (SARS) patients in the isolation intensive care unit (ICU).
