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Many seedlings showed very weak (Fig. 6c) to more intensive GUS expression in the central (vascular) tissue in the fused hypocotyl-cotyledon structure.
However, intensive GUS staining and higher free IAA contents were detected in the above-ground tissues of OE lines compared with WT and KO plants, indicating a significant alteration of the auxin distribution in OE plants.
Additionally, the exogenous IAA treatments (1 and 10 μM) not only suppressed GUS staining in the primary roots but also altered its distribution, with intensive GUS activity appearing in the lateral root primordium, indicating that pPLAIIIδ is involved in auxin-regulated lateral root development.
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