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After ethanol/formalin fixation and further permeabilization of the cells with 0.1% Triton X-100 the anti-K19M antibody (Protein A purified) gave an intensive granular fluorescence pattern which appeared perinuclear in a significant fraction of the cells with a similar pattern observed in H295R and G59/540 cells.
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Whisk every half-hour or so, until frozen and granular.
That's surveillance far more intensive, and more granular, than anything Walgreens or Bank of America will ever manage.
Despite intensive researches on granular architecture and strategies to improve treatment efficiency, there are still some elusive material parameters needed to stimulate the granulation process.
This realm of knowledge-intensive business services provides much more granular and targeted services than traditional forms of outsourcing.
A homogeneous cooling state for rods, where the time dependence of the system's intensive variables occurs only through a global granular temperature, has been identified.
In human cerebellum, intensive cell proliferation occurs within the external granular layer between the 28th and 34th gestational weeks and is still active by the fifth postnatal month (Abraham et al., 2001).
They are very labour intensive and have to be investigated at a granular lever to look at each trade, other trades going on at the same time and at market conditions".
In situ hybridization found high-level p53 mRNA expression during embryonic development and a striking p53 mRNA expression pattern in postnatal rat brain, with intensive signals in subventricular zone, rostral migratory stream and external granular layer (EGL), where new neurons were produced.
At the same time, the methodology allows us working with granular local-surrogate models when the process requires a more intensive search around a specific region of the search space.
The cells, forming neoplastic formation in which mitotic figures and intensive squeezed artefacts were found, were round or oval-shaped and having the granular chromatin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com