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Intensive annotation efforts were made only on the final 63.5K selected unique targets.
Most of the obtained sequences had no identical counterparts in databases and despite intensive annotation efforts (see Methods), 40%% of the ORFs were hypothetical proteins.
As expected because of our intensive annotation of the C. elegans genes, there were more setA proteins from that species, but many proteins from the other two species were also found.
The pathway for anaerobic benzene degradation has been partially deduced [ 5], but the enzymes responsible for this process have yet to be identified, and remain elusive even after the intensive annotation efforts described here-in.
In the museum model, a relatively small group of specialized curators perform a particular literature curation effort, while in the jamboree model a group of experts meet for a short intensive annotation workshop.
Party: the jamboree, a short intensive annotation workshop e.g. Drosophila, mouse cDNAs (7, 8 ) Cottage industry: decentralized effort among several groups of experts e.g. fungal and prokaryotic genomes (9 ) (10 ) Factory: automated genome analysis, used by the genome browsers [Ensembl (11 ), UCSC (12 ), NCBI mapViewer (13 )].
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We generated a high-quality, annotated onion transcriptome from de novo sequence assembly and intensive structural annotation using the integrated structural gene annotation pipeline (ISGAP), which identified 54,165 protein-coding genes among 165,179 assembled transcripts totalling 203.0 Mb by eliminating the intron sequences.
Only the Bovine and Chicken NetAffx annotations were richer than the ones generated by Blast2GO due to intensive proteome annotation efforts of GOA in collaboration with the International Protein Index (Barrell et al., 2009).
The predominant method for content-based tagging is through slow, labor-intensive manual annotation.
Investigating the origins of such phenomena requires detailed estimation of speech sound distributions; this has been hampered by reliance on subjective, labor-intensive manual annotation.
For C. elegans, the reference genome sequence is complete and our manual annotation was intensive enough to permit good estimates of the number of defective genes for each family, as summarized in Additional file 9.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com