Due to the varying surface reflectances and angles, pixels can have different photon intensities, which makes it a difficult problem to choose a reliable threshold.
Cheese fat and plant fat exhibit differences in fluorescence intensities, which makes it possible to distinguish between cheese and plant fats on the basis of their fluorescence spectra.
Although this normalization minimizes within-array spatial biases, there is still a difference in overall signal intensity between arrays, which makes it difficult to compare them.
The fact that both these states are long-lived states means that RESOLFT requires much lower laser intensities than STED, which makes it attractive for imaging biological samples over large areas or long times.
Even though solar radiation is known as the superior candidate, its density varies over time depending on many factors such as solar intensity and cloud states, which makes it difficult to predict and utilize the energy efficiently.
The Shannon-Weiner diversity index uses the total number and relative intensities of DGGE bands, which makes it more suitable to estimate bacterial diversity than the number of bands alone [ 45].
However it is not straightforward: firstly due to the inherent non-linearity of the MR signal intensity with respect to contrast agent concentration, which makes it impossible to compare different scans quantitatively.
Note, that mean signal was subtracted from time-series prior to all connectivity analyses (see Methods), which makes it unlikely that signal intensity differences affected the connectivity results".
The emission intensity of this complex changes in the pH range of 3 11, which makes it useful in the design of chemosensors.
