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Neuronal firing and early network oscillations were induced after a critical level of light intensity was reached.
After extensive washing with TBST, slides were incubated with streptavidin-HRP (1 500) for 15 min at RT, wash 3× with TBST and incubated with DAB substrate for 5 10 min at RT until desired color intensity was reached.
In the more analytically relevant region (afterglow), signals for 40Ar+ with this new design were negligible, while maximum intensity was reached earlier in time for 41(ArH)+ than when using the PROTOTYPE GD.
The highest rebound headache intensity was reached during the 2nd day of withdrawal.
When the desired band intensity was reached, the gel was fixed in pre-chilled acetic acid and dried for photography.
Further experiments using C medium with increasing glucose concentrations from 10 to 70 mM, indicated that, in all sugar-dependent biofilm formers, maximum crystal violet staining intensity was reached in wells where bacteria were grown at 30 mM or higher glucose concentrations (data not shown).
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After a few hours, an intermediate plateau in the integrated SANS intensity is reached and indicates an intermediate metastable state.
However, when the maximum intensity is reached for each PL peak is different.
The D 5 75 is calculated as the time interval at which 5 and 75% of the Arias intensity is reached.
In addition, the maximum intensity is reached when the annealing temperature is about 400°C which is a lower temperature than that of NAA fabricated under MA conditions in the same acid electrolyte (i.e. about 600°C) [11, 12].
Although the lack of band-edge emission makes it less clear than the ZnSxSe1−x case discussed above, the deep-trap emission (near 500 nm) from undesirable NCs shows similar trend of decreasing (although its size-dependent PL QY is clearly increasing, as shown in the undoped case in Additional file 1: Figure S7) prior to the maximum isolated Mn PL intensity being reached.
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