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The averaged data reveal that the intensity to generate a half-maximal amplitude response was significantly greater during the day than at night (Fig. 2A, Table 1).
The median background intensity was subtracted from the median spot intensity to generate the background-corrected signal intensity for use in further analysis.
Fluorescence was excited with ca. 100 fs light pulses (λexc = 760 nm) applied at sufficient intensity to generate two-photon excitation.
It is possible that there is not enough light intensity to generate sufficient amount of ATP required for cell growth in this region.
The background intensity was obtained for areas within circles of the same diameter in the vicinity of the spots and subtracted from the average intensity to generate the net intensity due to binding of the probe.
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The detection algorithm uses probe pair (includes perfect-match (PM) and mismatch (MM)) intensities to generate a detection p-value and assign a Present, Marginal, or Absent call.
Finally, a median polishing algorithm is used to summarize the background-corrected, normalized probe intensities to generate a final probe set value.
Finally, a median polishing algorithm was used to summarize the background-corrected and the normalized probe intensities to generate a final probe set value.
The detection algorithm uses probe pair intensities to generate a detection p-value and assign a "present", "marginal", or "absent" call.
Gene-level summarization algorithms, which combine multiple probe intensities to generate an overall expression estimate for the gene, can suffer from biases of probe cross-hybridization.
The second general application of ADV is to use the PFC droplet as a contrast agent, either in the liquid or gas form, to visualize and confirm the location of the desired delivery; then when or if the location is correct, the liquid or gas is subjected to higher intensity ultrasound to generate intense cavitational events that can disrupt carriers or cell membranes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com